Lifeact: a versatile marker to visualize F-actin (2024)

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References Acknowledgements Author information Authors and Affiliations Corresponding authors Ethics declarations Competing interests Supplementary information Supplementary Text and Figures Supplementary Movie 1 Supplementary Movie 2 Supplementary Movie 3 Supplementary Movie 4 Supplementary Movie 5 Supplementary Movie 6 Rights and permissions About this article Cite this article

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References

  1. Schmit, A.C. & Lambert, A.M. Plant Cell 2, 129–138 (1990).

    Article CAS Google Scholar

  2. Waterman-Storer, C.M., Desai, A., Bulinski, J.C. & Salmon, E.D. Curr. Biol. 8, 1227–1230 (1998).

    Article CAS Google Scholar

  3. Yamada, S., Pokutta, S., Drees, F., Weis, W.I. & Nelson, W.J. Cell 123, 889–901 (2005).

    Article CAS Google Scholar

  4. Edwards, K.A., Demsky, M., Montague, R.A., Weymouth, N. & Kiehart, D.P. Dev. Biol. 191, 103–117 (1997).

    Article CAS Google Scholar

  5. Bretschneider, T. et al. Curr. Biol. 14, 1–10 (2004).

    Article CAS Google Scholar

  6. Lenart, P. et al. Nature 436, 812–818 (2005).

    Article CAS Google Scholar

  7. Pang, K.M., Lee, E. & Knecht, D.A. Curr. Biol. 8, 405–408 (1998).

    Article CAS Google Scholar

  8. Burkel, B.M., von Dassow, G. & Bement, W.M. Cell Motil. Cytoskeleton 64, 822–832 (2007).

    Article CAS Google Scholar

  9. Asakura, T. et al. Oncogene 16, 121–130 (1998).

    Article CAS Google Scholar

  10. Yang, H.C. & Pon, L.A. Proc. Natl. Acad. Sci. USA 99, 751–756 (2002).

    Article CAS Google Scholar

  11. Cooper, J.A., Walker, S.B. & Pollard, T.D. J. Muscle Res. Cell Motil. 4, 253–262 (1983).

    Article CAS Google Scholar

  12. Czisch, M., Schleicher, M., Horger, S., Voelter, W. & Holak, T.A. Eur. J. Biochem. 218, 335–344 (1993).

    Article CAS Google Scholar

  13. Fischer, M., Haase, I., Wiesner, S. & Muller-Taubenberger, A. FEBS Lett. 580, 2495–2502 (2006).

    Article CAS Google Scholar

  14. McNeil, P.L., Murphy, R.F., Lanni, F. & Taylor, D.L. J. Cell Biol. 98, 1556–1564 (1984).

    Article CAS Google Scholar

  15. Tang, T. et al. J. Exp. Med. 186, 1853–1863 (1997).

    Article CAS Google Scholar

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Acknowledgements

We thank S. Uebel and the core facility of the Max Planck Institute of Biochemistry for peptide synthesis and labeling, and S. Cremer and S. Wedlich for helpful comments on the manuscript. We obtained latrunculin A from P. Crews (University of California Santa Cruz). This study was supported by funds from the Max Planck Society (R.W.-S.), German Research Foundation SI1323/1-1 (M.S.) and the Alexander von Humboldt Stiftung (Z.W.).

See Also
Lifeact: a versatile marker to visualize F-actinLifeact: a versatile marker to visualize F-actinLILAC: enhanced actin imaging with an optogenetic LifeactActin chromobody imaging reveals sub-organellar actin dynamics

Author information

Author notes

  1. Julia Riedl and Alvaro H Crevenna: These authors contributed equally to this work.

Authors and Affiliations

  1. Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, Martinsried, 82152, Germany

    Julia Riedl,Alvaro H Crevenna,Jerry Haochen Yu&Roland Wedlich-Soldner

  2. Department of Neuroimmunology, Max Planck Institute of Neurobiology, Am Klopferspitz 18, Martinsried, 82152, Germany

    Kai Kessenbrock&Dieter Jenne

  3. Max Planck Institute of Neurobiology, Independent Junior Research Group Axonal Growth and Regeneration, Am Klopferspitz 18, Martinsried, 82152, Germany

    Dorothee Neukirchen&Frank Bradke

  4. Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, 82152, Germany

    Michal Bista&Tad A Holak

  5. Department of Anatomy and The Biomedical Sciences Program, University of California, San Francisco, San Francisco, 513 Parnassus Avenue, 94143, California, USA

    Zena Werb

  6. Department of Molecular Medicine, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, 82152, Germany

    Michael Sixt

Authors

  1. Julia Riedl

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  2. Alvaro H Crevenna

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  3. Kai Kessenbrock

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  4. Jerry Haochen Yu

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  5. Dorothee Neukirchen

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  6. Michal Bista

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  7. Frank Bradke

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  8. Dieter Jenne

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  9. Tad A Holak

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  10. Zena Werb

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  11. Michael Sixt

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  12. Roland Wedlich-Soldner

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Corresponding authors

Correspondence to Michael Sixt or Roland Wedlich-Soldner.

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Competing interests

J.R., A.H.C., M.S. and R.W.-S. have submitted a patent application (07 023 084.2; Peptide for determining actin structures in living cells) to the EU patent office.

Supplementary information

Supplementary Text and Figures

Supplementary Figure 1, Supplementary Methods (PDF 193 kb)

Supplementary Movie 1

Actin dynamics in a mouse embryonic fibroblast. Images were acquired at 9 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. (MOV 1655 kb)

Supplementary Movie 2

Actin dynamics in a chemotactic mouse dendritic cell. Images were acquired at 3 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. (MOV 3004 kb)

Supplementary Movie 3

Dynamics of filopodia in a hippocampal neuron. Images were acquired at 2 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. (MOV 820 kb)

Supplementary Movie 4

Cortical actin dynamics in the cell body of a hippocampal neuron. Images were acquired at 2 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. (MOV 3804 kb)

Supplementary Movie 5

Cytokinetic ring formation during mitosis of MDCK cells. Images were acquired at 2 min/frame and are played back with 10 frames/s. Time stamp in hours and minutes. (MOV 238 kb)

Supplementary Movie 6

Spreading of a primary human neutrophil on a cover petri dish coated with immune complexes. Images were acquired at 1 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. (MOV 1832 kb)

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Riedl, J., Crevenna, A., Kessenbrock, K. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605–607 (2008). https://doi.org/10.1038/nmeth.1220

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  • DOI: https://doi.org/10.1038/nmeth.1220

Lifeact: a versatile marker to visualize F-actin (2024)
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